Formal ether sedimentation technique for the concentration of stool. Formal ether sedimentation technique for the concentration. A total of 447 stool samples collected at random from pupils in six different schools in djottin were examined for intestinal parasites using formolether, formolpetrol, and direct smear and floatation techniques. Free the plug of debris from the sides of the tube with an applicator stick. Accuracy of the katokatz method and formalinether concentration technique for the diagnosis of clonorchis sinensis, and implication for assessing drug efficacy menbao qian, 1 peiling yap, 2, 3 yichao yang, 4 hai liang, 5 zhihua jiang, 4 wei li, 5 jurg utzinger, 2, 3 xiaonong zhou, 1 and jennifer keiser 3, 6. Formalin acts both a fixative and preservative of protozoan eggs, larvae and cysts. Fecal specimens from selected schoolchildren and droppings of the vervet monkeys were collected and microscopically examined for intestinal parasites using the katokatz thick smear and formolether concentration. Formalin ether concentration method principle fecal concentration is a routine part of the ova and parasite examination and allows the. Therefore, formol ether concentration technique should be adopted for routine faecal examination since it exposes a higher percentage of infection missed by direct smear method, which is the implemented method by medical diagnostic laboratories and hospitals in developing countries e. This method is perhaps the most efficient concentration technique used in clinical laboratories for concentrating eggs from faeces. Out of the 76 persons who were both reference standard and sedimentation technique negative, there were 18 only positive with iha, 5 only with katokatz and 4 with a positive hatching test only. This method increases the sensivity as compare to direct wet mount with dobell iodine or normal saline. Although the concentration procedure destroys free form amoebae and cellular exudate, additional direct examinations offormed stools free ofblood and mucus. Using the formol ether concentration technique, 2 mg of stool sample was mixed with 7 ml of 10% formalsaline in a clean centrifuge tube.
The two most commonly used stool concentration techniques are. The number and percentage of infected cases with s. Comparison of the flotac400 dual technique and the formalin. Low sensitivity of the formolethyl acetate sedimentation. The highest prevalence of infections was recorded among the age group of 19 22 years 21% in both sexes, and least infection rate in the age group of 27 30.
A total of 103 samples collected from the same number of individuals were examined for intestinal parasites using the direct smear method and the formolether concentration technique. Formaldehyde commercial product 1200mls na2hpo4 10. The aim of this study was to evaluate the operational characteristics sensitivity and negative predictive value npv of wet mount, formol ether concentration fec, and katokatz techniques for the determination of intestinal parasitic infections. Comparison of the katokatz, wet mount, and formolether. The formolether concentration method uses 10% formalin as fixative, and ether is added for fat and debris extraction, followed by filtration and centrifugation. Methods to detect protozoa are needed for food safety monitoring. Miniflotac, an innovative direct diagnostic technique for. Fecal debris is extracted into the ether phase so that the parasitic forms can be separated and then sedimented by centrifugation. In one direct comparative study formol ether showed a significantly improved sensitivity for ascaris, hookworm and trichuris 58. More than 10 faecal examination techniques are known, including direct smear ds andor concentration tada et al. The simplified formolether concentration is carried out as follows.
Laboratory procedures for stool examination laboratory. There is a need for accurate diagnosis of intestinal parasite infections in humans, but currently available copromicroscopic techniques have shortcomings, such as low sensitivity. Evaluation of the efficacy of the mini parasep sf faecal. Diagnostic performance of direct wet mount microscopy in. The aim of this study was to evaluate the operational characteristics sensitivity and negative predictive value npv of wet mount, formolether concentration fec, and katokatz techniques for the determination of intestinal parasitic infections. Pdf miniflotac, an innovative direct diagnostic technique. This six minute video presents the formolether concentration technique used to examine fecal parasites. The most effective method of coprodetection of strongyloides stercoralis larvae is the agar plate culture apc method as it is 1. Fecal parasite examination the formolether concentration. Comparison of the flotac400 dual technique and the. Formol ethyl acetate sedimentation concentration is a technique for detecting eggs in stool by microscopy, but has not to our knowledge been evaluated for s. A prevalence survey of enteral parasites in preschool. Safan alternative fixation solution for parasitological stool specimens. Pdf we compared the efficacy and applicability of a modified formalinether.
Mix a small portion of the stool, about the size of a marble, in 10 ml 5% 10% formalin or saline in a flatbottomed waxed paper cup, carton, or 16x 125 mm test tube. In particular, the formalin ether sedimentation technique 406th medical general laboratory mgl, first reported by ritchie 1948, is commonly used for detecting helminth eggs, larvae, and. Different studies showed that formol ether concentration technique fec is more sensitive than the conventional direct wet mount microscopy. The efficacy of mini parasep, a new faecal parasite concentrator developed by the company diasys europe limited formerly intersep ltd was evaluated alongside the modified formol ether concentration and direct smear techniques using 120 stool samples in calabar, nigeria between may and june, 2011.
Therefore, the employment of fec techniques as a confirmatory test in routine laboratory examination of stool will significantly aid in accurate determination and management of parasitic infections 7. A total of 354 faecal specimens were collected from students in northwest ethiopia and screened with katokatz, wet mount. Formol ether concentration technique was used to analyze the stool samples. Formolethyl acetate sedimentation concentration is a technique for detecting eggs in stool by microscopy, but has not to our knowledge been evaluated for s. The technique recommended for the qualitative diagnosis of intestinal parasites both helminths and intestinal protozoa is the formol ether concentration method fecm, performed on three different samples, but the direct fecal smear on a single sample is used as diagnostic method more often in resourceconstrained settings. Here we describe an alternative, cheap, rapid, simple methods for analyzing fresh produce cabbage, lettuce for contamination with giardia cysts elution followed by formol ether concentration followed by detection by pcr. Stool concentration techniques, especially formol formalin ether or formol ethyl acetate sedimentation concentration is the preferred method for detection of helminth eggs and protozoa cysts in many clinical parasitology laboratories, although to a lesser extent in africa and asia. The formalinether concentration technique fect is a widely used sedimentation technique. Free the plug of debris from the side of the tube by ringing with an applicator. Apr 12, 2018 formol ether sedimentation technique article available in pdf the use of concentration techniques increases the chances of identifying intestinal parasitic wether helminths or protozoans. A semi structured interviewer administered questionnaire was used to collect information from participants.
Further observations on the formolether concentration technique for faecal parasites. Formalin ether concentration technique laboratory stack. Schistosoma mansoni and other intestinal parasitic. Miranda questions for research update brief summary draw and label. Strongyloides stercoralis, formalin ether concentration technique, agar plate culture, feces, diagnosis in tropical and subtropical areas, strongyloidiasis is a widespread human parasitic disease that can be lifethreatening to immunocompromised hosts as well as patients undergoing corticosteroid treatment or chemotherapy 1. Modified formalinether concentration technique for. J clin pathol online june 16, 2011 preservative used 28% 26% 23% 1% 1% 21% formalin in water formalin in saline. The sedimentation technique used at cdc is the formalinethyl acetate technique, a diphasic sedimentation technique that avoids the problems of flammability of ether, and which can be used with specimens preserved in formalin, mif or saf. Stool samples were collected processed with formol ether concentration technique and examined for viable eggs of ascaris.
Different studies showed that formolether concentration technique fec is more sensitive than the conventional direct wet mount microscopy. Preparation of giardia cysts to evaluate the concentration methods. Formol ether concentration method in the diagnosis of active schistosoma mansoni in patients with detectable iha. Formolether concentration method in the diagnosis of active. Pdf a modified formalinether concentration technique.
Four layers formed after the second centrifugation of the fact improved diagnosis of strongyloides stercoralis using recombinant antigenbased. Clonorchiasis is a chronic neglected disease caused by a liver fluke, clonorchis sinensis. The most commonly used fixatives for stool preservation are either formalin or sodium acetateacetic acidformalin saf 3, 24, 40. Other techniques such as mcmaster, flotac and formolether concentration fec are also available to diagnose sths infections. Formolether concentration technique was used to analyze the stool samples. We assessed the diagnostic accuracy of the katokatz method and the formalinether concentration technique fect for c. Formal ether sedimentation technique for the concentration of. Fecal parasite examination the formolether concentration multi.
Further observations on the formolether concentration. Formolether concentration method in the diagnosis of. The labourintensive procedure and hazards associated with the use of inflammable lipid solvents have encouraged the development of commercial products such as parasep which is a. The prepared slides and preserved specimens were transported to the medical parasitology laboratory of the aklilu lemma institute of pathobiology, addis ababa university, within four to five days of specimen collection. Formol ether concentration method is the sedimentation technique normally used for detecting parasite infections, especially eggs and protozoan cysts with a high fat in stool samples. Here we describe an alternative, cheap, rapid, simple methods for analyzing fresh produce cabbage, lettuce for contamination with giardia cysts elution followed by formolether concentration followed by detection by pcr. The specific gravity of protozoan cysts and helminth eggs is greater than that of water.
Principle of formal ether formalinethyl aceatate sedimentation technique. Workers and seafarers will adapt the formalin ether concentration technique fect. Modified formalinether concentration technique for diagnosis of. Free the plug of debris from the top of the tube by ringing the sides with an. Jan 01, 2018 techniques\cases direct stool examination formol ether concentration present 22 23. The formalin ether concentration technique fect is a widely used sedimentation technique for the diagnosis of intestinal protozoa in preserved stool samples. Comparison of methods for detecting blastocystis hominis. Mohammad a al mofarreh 1, ahmad m al akwaa 2, sayed w hasan 1, ibrahim a al mofleh 2 1 dr. A total of 354 faecal specimens were collected from students in northwest ethiopia and screened with katokatz. Dec 23, 2010 this six minute video presents the formol ether concentration technique used to examine fecal parasites. Formal ether sedimentation technique for the concentration of stool parasites. Sedimentation techniques use solutions of lower specific gravity than the parasitic organisms, thus concentrating the latter in the sediment.
The overall prevalence of ascaris infection found in this. Using the formolether concentration technique, 2 mg of stool sample was mixed with 7 ml of 10% formalsaline in a clean centrifuge tube. Formalin ether concentration technique formalin ether concentration technique is a method use to concentrate parasitic elements through sedimentation to enhance recovery. Jcdr concentration techniques, simple salt floatation. Mix the contents of the vial by shaking vigorously or vortexing for 30 seconds.
Formalinether sedimentation concentration definition of. An accurate diagnosis is important in order to identify individuals and communities in need of intervention, and for monitoring drug efficacy and potential emergence of resistance. This resource was developed by charles adjei osei of the kwame nkrumah university of. According to ritchie 1948, the recovery efficiency of the mgl technique for. This video demonstrates both the sedimentation formolether and flotation zinc sulfate methods of stool feces concentration. Prevalence of human intestinal helminth parasites among. Comparison of the katokatz method and etherconcentration. Free the plug of debris from the side of the tube by ringing with an applicator stick. Pdf the formolether concentration technique for intestinal. The technique recommended for the qualitative diagnosis of intestinal parasites both helminths and intestinal protozoa is the formolether concentration method fecm, performed on three different samples, but the direct fecal smear on a single sample is used as diagnostic method more often in resourceconstrained settings. Oct 29, 20 clonorchiasis is a chronic neglected disease caused by a liver fluke, clonorchis sinensis.
Soiltransmitted helminth infections are a major public health problem. The formolether method is a widely used technique for stool examination. Diagnostic performance of mini parasep solventfree faecal. Add 50ml of diethyt ether, shake well and leave for 10min, shaking. This six minute video presents the formol ether concentration technique used to examine fecal parasites. We compared the technique, using a single stool sample and a commercial preparation kit, with three tests traditionally used in the endemic countries katokatz thick smear. Katokatz is the method favoured by the who in prevalence studies, however formol ether concentration holds up well against kato katz in previous analysis 20, 21. Diethyl ether commercial product, pml 10% neutral buffered formalin ph 7. Suspend the residue in 100 ml of formol saline solution 100 ml 40% hcho solu tion, 9 g nac11 and rinse into a 500 ml conical flask. Al mofarreh polyclinic, kkuh, riyadh, saudi arabia 2 department of medicine, kkuh, riyadh, saudi arabia click here for correspondence address.
The mixture was then filtered through doublelayered cotton gauze into a clinical flask and 3 ml of diethyl ether was added and the mixture was vigorously shaken. Chemotherapy is the mainstay of control and treatment efficacy is usually determined by microscopic examination of fecal samples. We compared the accuracy of the katokatz method and ether concentration technique for the diagnosis of soiltransmitted helminth infections. Formol ether concentration technique is taken as a gold standard method to detect most intestinal parasites. Diagnostic performance of mini parasep solventfree foecal. Strongyloides stercoralis, formalinether concentration. To evaluate a formol acetone concentration method in comparison with the conventional direct iodine preparation and formol ether concentration methods. Transmission of schistosoma mansoni in yachi areas. The sedimentation technique used at cdc is the formalinethyl acetate.
Nov 23, 20 soiltransmitted helminth infections are a major public health problem. Formalinether concentration method principle fecal concentration is a routine part of the ova and parasite examination and allows the. Bukumbi for intestinal parasitic infections with three diagnostic methods. Modified formalinether concentration technique for diagnosis. Laboratory procedures for stool examination issued by. To assess schistosoma mansoni and other intestinal parasitic infections in schoolchildren and vervet monkeys chlorocebus aethiops in bochessa village, ziway, ethiopia. We compared the accuracy of the katokatz method and etherconcentration technique for the diagnosis of soiltransmitted helminth. Request pdf the formolether concentration technique for intestinal parasites. Formal ether sedimentation stool concentration technique. The organism was detected in 39 of the 1,000 samples investigated using the in vitro culture technique and in none of the samples using the formolether concentration technique. Pdf a modified formalinether concentration technique for. With a pipette, mix the remaining sediment with the small amount or remaining fluid and transfer one drop each to a drop of saline and.
Prevalence of intestinal schistosomiasis among basic. With the 50 ml centrifuge vial still loosely attached to the filter unit loose attachment will facilitate the release of air pressure during use, insert the open en d of the filter unit into the specimen vial until the. Miniflotac, an innovative direct diagnostic technique for intestinal parasitic infections. At the end of the study, free treatment with albendazole 400 mg was offered to all study participants. We compared the diagnostic accuracy of a further modified version of the recently developed flotac technique with that of the widely used formalinether concentration technique fect for the detection. We assessed the diagnostic accuracy of the katokatz method and the formalin ether concentration technique fect for c. Mar 31, 2011 this video demonstrates both the sedimentation formol ether and flotation zinc sulfate methods of stool feces concentration. A modified formalin ether concentration technique for diagnosis of human strongyloidiasis article pdf available in the korean journal of parasitology 516. Fecal debris is extracted into the ether phase so that the ethyl acetate formalin 510% nss. The value formolether concentration of faecal cysts. Formolether concentration method in the diagnosis of active schistosoma mansoni in patients with detectable iha. Strongyloides stercoralis, formalinether concentration technique, agar plate culture, feces. The sediment was used for the parasitic examination. The first page of the pdf of this article appears above.
Prevalence of intestinal schistosomiasis among basic school. The manufacturers instruction was strictly followed in the evaluation of the new test while. Experience from the field plos neglected tropical diseases, aug 20 beatrice divina barda, laura rinaldi, davide ianniello, henry zepherine, fulvio salvo, tsetan sadutshang, giuseppe cringoli, massimo clementi. Unfortunately, the formolether methodsuffers fromthe failing ofmostconcentration procedures, that free form amoebae and cellular exudateare destroyed. The study showed that infections were more prevalent among males.
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